5,0
8 sept. 2014
Employé (ancien), plus de 5 an(s)
Union Beach, NJ
Recommande
Approbation du PDG
Perspective commerciale
Avantages
IFF brought the new concepts of cell-based assay to taste modulation compound screening, and created a lot of new modulators for sweet, umami and bitter taste qualities.
Inconvénients
Creation of taste receptor cell lines and evaluation was the biggest challenge since all sweet and umami receptors with at least two subunits and a G-protein, so it was hard to create a cell line with good response in taste assay, especially in HTS evaluation. I tried different vectors, different vector combinations, and three gene in separate vectors or three genes in one vector, finally I got some working stable cell lines. From this, I got a lot of experience in how to create a good stable cell lines and how to evaluate them.